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原著論文 / Original Articles

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2019

  1. Serizawa T, Isotani A, Matsumura T, Nakanishi K, Nonaka S, Shibata S, Ikawa M, Okano H.
    Developmental analyses of mouse embryos and adults using a non-overlapping tracing system for all three germ layers.
    Development 2019 Nov 4;146(21). pii: dev174938.

  2. §Matsumura T, §Noda T, Muratani M, Okada R, Yamane M, Isotani A, Kudo T, Takahashi S, Ikawa M.
    §: equal contribution
    Male mice, caged in the International Space Station for 35 days, sire healthy offspring.
    Sci Rep. in press
    微研プレスリリース

  3. Kiyozumi D, Mori M, Kodani M, Ikawa M.
    Genetic mutation of Frem3 does not cause Fraser syndrome in mice.
    Exp Anim 2019 Sep 26. [Epub ahead of print]

  4. §Fujihara Y, §Noda T, §Kobayashi K, Oji A, Kobayashi S, Matsumura T, Larasati T, Oura S, Kojima-Kita K, Yu Z, Matzuk MM, Ikawa M.
    §: equal contribution
    Identification of multiple male reproductive tract-specific proteins that regulate sperm migration through the oviduct in mice.
    PNAS 2019 Sep 10;116(37):18498-18506.
    https://www.ncbi.nlm.nih.gov/pubmed/31455729
    微研プレスリリース

  5. Holcomb RJ, Oura S, Nozawa K, Kent K, Yu Z, Robertson MJ, Coarfa C, Matzuk MM, Ikawa M, Garcia TX.
    The testis-specific serine proteases Prss44, Prss46, and Prss54 are dispensable for mouse fertility.
    Biol Reprod.2019 Aug 12. Epub ahead of print.
    https://www.ncbi.nlm.nih.gov/pubmed/31403672

  6. Hayashi M, Machida T, Ishida Y, Ogata Y, Omori T, Takasumi M, Endo Y, Suzuki T, Sekimata M, Homma Y, Ikawa M, Ohira H, Fujita T, Sekine H.
    Cutting Edge: Role of MASP-3 in the Physiological Activation of Factor D of the Alternative Complement Pathway.
    J Immunol. 2019 Sep 15;203(6):1411-1416.
    https://www.ncbi.nlm.nih.gov/pubmed/31399515

  7. Sasaki K, Shiba K, Nakamura A, Kawano N, Satouh Y, Yamaguchi H, Morikawa M, Shibata D, Yanase R, Jokura K, Nomura M, Miyado M, Takada S, Ueno H, Nonaka S, Baba T, Ikawa M, Kikkawa M, Miyado K, Inaba K.
    Calaxin is required for cilia-driven determination of vertebrate laterality.
    Commun Biol. 2019 Jun 20;2:226.
    https://www.ncbi.nlm.nih.gov/pubmed/31240264
    筑波プレスリリース

  8. Lu Y, Oura S, Matsumura T, Oji A, Sakurai N, Fujihara Y, Shimada K, Miyata H, Tobita T, Noda T, Castaneda JM, Kiyozumi D, Zhang Q, Larasati T, Young SAM, Kodani M, Huddleston CA, Robertson MJ, Coarfa C, Isotani A, Aitken RJ, Okabe M, Matzuk MM, Garcia TX, Ikawa M.
    CRISPR/Cas9-mediated genome editing reveals 30 testis-enriched genes dispensable for male fertility in mice.
    Biol Reprod. 2019 Aug 1;101(2):501-511.
    https://www.ncbi.nlm.nih.gov/pubmed/31201419

  9. Shiromoto Y, Kuramochi-Miyagawa S, Nagamori I, Chuma S, Arakawa T, Nishimura T, Hasuwa H, Tachibana T, Ikawa M, Nakano T.
    GPAT2 is required for piRNA biogenesis, transposon silencing, and maintenance of spermatogonia in mice.
    Biol Reprod. 2019 Apr 5. pii: ioz056.
    https://www.ncbi.nlm.nih.gov/pubmed/30951587

  10. Shide K, Kameda T, Kamiunten A, Oji A, Ozono Y, Sekine M, Honda A, Kitanaka A, Akizuki K, Tahira Y, Nakamura K, Hidaka T, Kubuki Y, Abe H, Miike T, Iwakiri H, Tahara Y, Sueta M, Hasuike S, Yamamoto S, Nagata K, Ikawa M, Shimoda K.
    Mice with Calr mutations homologous to human CALR mutations only exhibit mild thrombocytosis.
    Blood Cancer J. 2019 Mar 29;9(4):42.
    https://www.ncbi.nlm.nih.gov/pubmed/30926777

  11. §Noda T, §Sakurai N, Nozawa K, Kobayashi S, Devlin DJ, Matzuk MM, Ikawa M.
    §: equal contribution
    Nine genes abundantly expressed in the epididymis are not essential for male fecundity in mice.
    Andrology. 2019 Sep;7(5):644-653.
    https://www.ncbi.nlm.nih.gov/pubmed/30927342

  12. Tobita T, Kiyozumi D, Muto M, Noda T, Ikawa M.
    Lvrn expression is not critical for mouse placentation.
    J Reprod Dev. 2019 Jun 14;65(3):239-244.
    https://www.ncbi.nlm.nih.gov/pubmed/30745494

  13. Noda T, Fujihara Y, Matsumura T, Oura S, Kobayashi S, Ikawa M.
    Seminal vesicle secretory protein 7, PATE4, is not required for sperm function but for copulatory plug formation to ensure fecundity.
    Biol Reprod.2019 Apr 19;100(4):1035-1045
    https://www.ncbi.nlm.nih.gov/pubmed/30452524

  14. Shimada K, Kato H, Miyata H, Ikawa M.
    Glycerol kinase 2 is essential for proper arrangement of crescent-like mitochondria to form the mitochondrial sheath during mouse spermatogenesis.
    J Reprod Dev. 2019 Apr 12;65(2):155-162.
    https://www.ncbi.nlm.nih.gov/pubmed/30662012

  15. Kusakabe S, Suzuki T, Sugiyama Y, Haga S, Horike K, Tokunaga M, Hirano J, He Z, Chen DV, Ishiga H, Komoda Y, Ono C, Fukuhara T, Yamamoto M, Ikawa M, Satoh T, Akira S, Tanaka T, Moriishi K, Fukai M, Taketomi A, Yoshio S, Kanto T, Suzuki T, Okamoto T, Matsuura Y.
    USP15 participates in HCV propagation through the regulation of viral RNA translation and lipid droplet formation.
    J Virol. 2019 Mar 5;93(6). pii: e01708-18.
    https://www.ncbi.nlm.nih.gov/pubmed/30626683

  16. Matsumura T, Endo T, Isotani A, Ogawa M, Ikawa M.
    An Azoospermic Factor gene, Ddx3y and its paralog, Ddx3x are dispensable in germ cells for male fertility.
    J Reprod Dev. 2019 Apr 12;65(2):121-128.
    https://www.ncbi.nlm.nih.gov/pubmed/30613052

  17. Senoo M, Takijiri T, Yoshida N, Ozawa M, Ikawa M.
    PTBP1 contributes to spermatogenesis through regulation of proliferation in spermatogonia.
    J Reprod Dev. 2019 Feb 8;65(1):37-46.
    https://www.ncbi.nlm.nih.gov/pubmed/30416150

  18. Oura S, Miyata H, Noda T, Shimada K, Matsumura T, Morohoshi A, Isotani A, Ikawa M.
    Chimeric analysis with newly established EGFP/DsRed2-tagged ES cells identify HYDIN as essential for spermiogenesis in mice.
    Exp Anim. 2019 Feb 26;68(1):25-34.
    https://www.ncbi.nlm.nih.gov/pubmed/30089752

  19. Kimura H, Yamamoto H, Harada T, Fumoto K, Osugi Y, Sada R, Maehara N, Hikita H, Mori S, Eguchi H, Ikawa M, Takehara T, Kikuchi A.
    CKAP4, a DKK1 receptor, is a biomarker in exosomes derived from pancreatic cancer and a molecular target for therapy.
    Clin Cancer Res. 2019 Jan 4. pii: clincanres.2124.2018.
    https://www.ncbi.nlm.nih.gov/pubmed/30610103

  20. Sato Y, Kiyozumi D, Futaki S, Nakano I, Shimono C, Kaneko N, Ikawa M, Okabe M, Sawamoto K, Sekiguchi K.
    Ventricular-subventricular zone fractones are speckled basement membranes that function as a neural stem cell niche.
    Mol Biol Cell. 2019 Jan 1;30(1):56-68.
    https://www.ncbi.nlm.nih.gov/pubmed/30379609

  21. 2018

    1. Carvajal G, Brukman NG, Weigel Muñoz M, Battistone MA, Guazzone VA, Ikawa M, Haruhiko M, Lustig L, Breton S, Cuasnicu PS.
      Impaired male fertility and abnormal epididymal epithelium differentiation in mice lacking CRISP1 and CRISP4.
      Sci Rep. 2018 Dec 3;8(1):17531.
      https://www.ncbi.nlm.nih.gov/pubmed/30510210

    2. Kiyozumi D, Taniguchi Y, Nakano I, Toga J, Yagi E, Hasuwa H, Ikawa M, Sekiguchi K.
      Laminin γ1 C-terminal Glu to Gln mutation induces early postimplantation lethality.
      Life Sci Alliance. 2018 Sep 10;1(5):e201800064.
      https://www.ncbi.nlm.nih.gov/pubmed/30456378

    3. Suzuki T, Okamoto T, Katoh H, Sugiyama Y, Kusakabe S, Tokunaga M, Hirano J, Miyata Y, Fukuhara T, Ikawa M, Satoh T, Yoshio S, Suzuki R, Saijo M, Huang DCS, Kanto T, Akira S, Matsuura Y.
      Infection with flaviviruses requires BCLXL for cell survival.
      PLoS Pathog. 2018 Sep 27;14(9):e1007299.
      https://www.ncbi.nlm.nih.gov/pubmed/30261081

    4. Sakamoto S, Thumkeo D, Ohta H, Zhang Z, Huang S, Kanchanawong P, Fuu T, Watanabe S, Shimada K, Fujihara Y, Yoshida S, Ikawa M, Watanabe N, Saitou M, Narumiya S.
      mDia1/3 generate cortical F-actin meshwork in Sertoli cells that is continuous with contractile F-actin bundles and indispensable for spermatogenesis and male fertility.
      PLoS Biol. 2018 Sep 26;16(9):e2004874.
      https://www.ncbi.nlm.nih.gov/pubmed/30256801

    5. Fujihara Y, Oji A, Kojima-Kita K, Larasati T, Ikawa M.
      Co-expression of sperm membrane proteins CMTM2A and CMTM2B is essential for ADAM3 localization and male fertility in mice.
      J Cell Sci.2018 Oct 8;131(19). pii: jcs221481.
      https://www.ncbi.nlm.nih.gov/pubmed/30209135
      Research Highlightとして紹介

    6. Abbasi F, Miyata H, Shimada K, Morohoshi A, Nozawa K, Matsumura Y, Xu Z, Pratiwi P, Ikawa M.
      RSPH6A is required for sperm flagellum formation and male fertility in mice.
      J Cell Sci. 2018 Oct 11;131(19).
      https://www.ncbi.nlm.nih.gov/pubmed/30185526

    7. Nishimasu H, Shi X, Ishiguro S, Gao L, Hirano S, Okazaki S, Noda T, Abudayyeh OO, Gootenberg JS, Mori H, Oura S, Holmes B, Tanaka M, Seki M, Hirano H, AburataniH, Ishitani R, Ikawa M, Yachie N, Zhang F, Nureki O.
      Engineered CRISPR-Cas9 nuclease with expanded targeting space.
      Science. 2018 Aug 30. pii: eaas9129.
      https://www.ncbi.nlm.nih.gov/pubmed/30166441

    8. Aoki A, Nakashima A, Kusabiraki T, Ono Y, Yoshino O, Muto M, Kumasawa K, Yoshimori T, Ikawa M, Saito S.
      Trophoblast-Specific Conditional Atg7 Knockout Mice Develop Gestational Hypertension.
      Am J Pathol. 2018 Aug 27. pii: S0002-9440(18)30366-3.
      https://www.ncbi.nlm.nih.gov/pubmed/30165042

    9. Fustin JM, Kojima R, Itoh K, Chang HY, Ye S, Zhuang B, Oji A, Gibo S, Narasimamurthy R, Virshup D, Kurosawa G, Doi M, Manabe I, Ishihama Y, Ikawa M, Okamura H.
      Two Ck1δ transcripts regulated by m6A methylation code for two antagonistic kinases in the control of the circadian clock.
      Proc Natl Acad Sci U S A. Jun 5;115(23):5980-5985.
      https://www.ncbi.nlm.nih.gov/pubmed/29784786

    10. Tanaka T, Shimizu S, Ueno M, Fujihara Y, Ikawa M, Miyata S.
      MARCKSL1 Regulates Spine Formation in the Amygdala and Controls the Hypothalamic-Pituitary-Adrenal Axis and Anxiety-Like Behaviors.
      EBioMedicine. Apr;30:62-73.
      https://www.ncbi.nlm.nih.gov/pubmed/29580842

    11. Murase M, Kawasaki T, Hakozaki R, Sueyoshi T, Putri DDP, Kitai Y, Sato S, Ikawa M, Kawai T.
      Intravesicular Acidification Regulates Lipopolysaccharide Inflammation and Tolerance through TLR4 Trafficking.
      J Immunol. Apr 15;200(8):2798-2808.
      https://www.ncbi.nlm.nih.gov/pubmed/29540576

    12. Shibata T, Shibata S, Ishigaki Y, Kiyokawa E, Ikawa M, Singh DP, Sasaki H, Kubo E.
      Tropomyosin 2 heterozygous knockout in mice using CRISPR-Cas9 system displays the inhibition of injury-induced epithelial-mesenchymal transition, and lens opacity.
      Mech Ageing Dev. Apr;171:24-30.
      https://www.ncbi.nlm.nih.gov/pubmed/29510160

    13. Fujita T, Kitaura F, Oji A, Tanigawa N, Yuno M, Ikawa M, Taniuchi I, Fujii H.
      Transgenic mouse lines expressing the 3xFLAG-dCas9 protein for enChIP analysis.
      Genes Cells. Apr;23(4):318-325.
      https://www.ncbi.nlm.nih.gov/pubmed/29480524

    14. Nozawa K, Satouh Y, Fujimoto T, Oji A, Ikawa M.
      Sperm-borne phospholipase C zeta-1 ensures monospermic fertilization in mice.
      Sci Rep. Jan 22;8:1315.
      https://www.ncbi.nlm.nih.gov/pubmed/29358633

    15. 2017

      1. Abe S, Kobayashi K, Oji A, Sakuma T, Kazuki T, Takehara S, Nakamura K, Okada A, Tsukazaki Y, Senda N, Honma K, Yamamoto T, Ikawa M, Chiba K, Oshimura M, Kazuki Y.
        Modification of single-nucleotide polymorphism in a fully humanized CYP3A mouse by genome editing technology.
        Sci Rep. 2017 Nov 9;7(1):15189
        https://www.ncbi.nlm.nih.gov/pubmed/29123154

      2. Fujihara Y, Oji A, Larasati T, Kojima-Kita K, Ikawa M.
        Human Globozoospermia-Related Gene Spata16 Is Required for Sperm Formation Revealed by CRISPR/Cas9-Mediated Mouse Models.
        Int J Mol Sci. Oct 21;18(10):2208.
        https://www.ncbi.nlm.nih.gov/pubmed/29065458

      3. Mashiko D, Ikawa M, Fujimoto K.
        Mouse spermatozoa with higher fertilization rates have thinner nuclei.
        PeerJ 2017 Oct 5:e3913.
        https://www.ncbi.nlm.nih.gov/pubmed/29038763

      4. Honda M, Hidaka K, Fukada SI, Sugawa R, Shirai M, Ikawa M, Morisaki T.
        Vestigial-like 2 contributes to normal muscle fiber type distribution in mice.
        Sci Rep. Aug 2;7(1):7168.
        https://www.ncbi.nlm.nih.gov/pubmed/28769032

      5. Isotani A, Matsumura T, Ogawa M, Tanaka T, Yamagata K, Ikawa M, Okabe M.
        A delayed sperm penetration of cumulus layers by disruption of acrosin gene in rats.
        Biol Reprod. 2017 Jul 1;97(1):61-68.
        https://www.ncbi.nlm.nih.gov/pubmed/28859281

      6. Castaneda JM, Hua R, Miyata H, Oji A, Guo Y, Cheng Y, Zhou T, Guo X, Cui Y, Shen B, Wang Z, Hu Z, Zhou Z, Sha J, Prunskaite-Hyyrylainen R , Yu Z, Ramirez-Solis R, Ikawa M, Matzuk MM and Liu M
        TCTE1 is a conserved component of the dynein regulatory complex and is required for motility and metabolism in mouse spermatozoa.
        Proc Natl Acad Sci U S A. 2017 Jul 3;114(27):E5370-E5378.
        https://www.ncbi.nlm.nih.gov/pubmed/28630322
        微生物病研究所 「主な研究成果」 紹介ページへ

      7. Ishimoto K, Ikawa M. & Okabe, M.
        The mechanics clarifying counterclockwise rotation in most IVF eggs in mice.
        Sci Rep. 2017 Mar 3;7:43456.
        https://www.ncbi.nlm.nih.gov/pubmed/?term=28256541

      8. Satouh Y, Nozawa K, Yamagata K, Fujimoto T, Ikawa M.
        Viable offspring after imaging of Ca2+ oscillations and visualization of the cortical reaction in mouse eggs.
        Biol Reprod. 2017 Mar 1;96(3):563-575.
        https://www.ncbi.nlm.nih.gov/pubmed/28339615

      9. Naruse C, Shibata S, Tamura M, Kawaguchi T, Abe K, Sugihara K, Kato T, Nishiuchi T, Wakana S, Ikawa M, Asano M.
        New insights into the role of Jmjd3 and Utx in axial skeletal formation in mice.
        FASEB J. 2017 Jun;31(6):2252-2266.
        https://www.ncbi.nlm.nih.gov/pubmed/28188179
        外部紹介ページへ

      10. Onda K, Tong S, Beard S, Binder N, Muto M, Senadheera SN, Parry L, Dilworth M, Renshall L, Brownfoot F, Hastie R, Tuohey L, Palmer K, Hirano T, Ikawa M, Kaitu'u-Lino T, Hannan NJ.
        Proton Pump Inhibitors Decrease Soluble fms-Like Tyrosine Kinase-1 and Soluble Endoglin Secretion, Decrease Hypertension, and Rescue Endothelial Dysfunction.
        Hypertension. 2017 Mar;69(3):457-468.
        http://www.ncbi.nlm.nih.gov/pubmed/28115513

      11. Fujimoto K, Kinoshita M, Tanaka H, Okuzaki D, Shimada Y, Kayama H, Okumura R, Furuta Y, Narazaki M, Tamura A, Hatakeyama S, Ikawa M, Tsuchiya K, Watanabe M, Kumanogoh A, Tsukita S, Takeda K.
        Regulation of intestinal homeostasis by the ulcerative colitis-associated gene RNF186.
        Mucosal Immunol. 2017 Mar;10(2):446-459.
        http://www.ncbi.nlm.nih.gov/pubmed/27381925

      12. Ueda J, Harada A, Urahama T, Machida S, Maehara K, Hada M, Makino Y, Nogami J, Horikoshi N, Osakabe A, Taguchi H, Tanaka H, Tachiwana H, Yao T, Yamada M, Iwamoto T, Isotani A, Ikawa M, Tachibana T, Okada Y, Kimura H, Ohkawa Y, Kurumizaka H, Yamagata K.
        Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis.
        Cell Rep. 2017 Jan 17;18(3):593-600.
        http://www.ncbi.nlm.nih.gov/pubmed/28099840

      13. Ishikawa T, Kitaura Y, Kadota Y, Morishita Y, Ota M, Yamanaka F, Xu M, Ikawa M, Inoue N, Kawano F, Nakai N, Murakami T, Miura S, Hatazawa Y, Kamei Y, Shimomura Y.
        Muscle-specific deletion of BDK amplifies loss of myofibrillar protein during protein undernutrition.
        Sci Rep. 2017 Jan 4;7:39825.
        http://www.ncbi.nlm.nih.gov/pubmed/28051178

      14. 2016

        1. Lee GH, Fujita M, Takaoka K, Murakami Y, Fujihara Y, Kanzawa N, Murakami KI, Kajikawa E, Takada Y, Saito K, Ikawa M, Hamada H, Maeda Y, Kinoshita T.
          A GPI processing phospholipase A2, PGAP6, modulates Nodal signaling in embryos by shedding CRIPTO.
          J Cell Biol. 2016 Dec 5;215(5):705-718.
          http://www.ncbi.nlm.nih.gov/pubmed/27881714

        2. Young SA, Miyata H, Satouh Y, Aitken RJ, Baker MA, Ikawa M.
          CABYR is essential for fibrous sheath integrity and progressive motility in mouse spermatozoa.
          J Cell Sci. 2016 Dec 1;129(23):4379-4387.
          http://www.ncbi.nlm.nih.gov/pubmed/27802166

        3. Young SA, Miyata H, Satouh Y, Muto M, Larsen MR, Aitken RJ, Baker M, Ikawa M.
          CRISPR/Cas9-mediated mutation revealed cytoplasmic tail is dispensable for IZUMO1 function and male fertility.
          Reproduction.2016 Dec;152(6):665-672.
          http://www.ncbi.nlm.nih.gov/pubmed/27624483

        4. Takashima K, Takeda Y, Oshiumi H, Shime H, Okabe M, Ikawa M, Matsumoto M, Seya T.
          STING in tumor and host cells cooperatively work for NK cell-mediated tumor growth retardation.
          Biochem Biophys Res Commun.2016 Sep 30;478(4):1764-71.
          http://www.ncbi.nlm.nih.gov/pubmed/27608599

        5. Oji A, Noda T, Fujihara Y, Miyata H, Kim YJ, Muto M, Nozawa K, Matsumura T, Isotani A, Ikawa M. CRISPR/Cas9 mediated genome editing in ES cells and its application for chimeric analysis in mice.Sci Rep. 2016 Aug 17;6:31666. http://www.ncbi.nlm.nih.gov/pubmed/27530713

        6. Kato K, Satouh Y, Nishimasu H, Kurabayashi A, Morita J, Fujihara Y, Oji A, Ishitani R, Ikawa M, Nureki O.
          Structural and functional insights into IZUMO1 recognition by JUNO in mammalian fertilization.
          Nat Commun. 2016 Jul 15;7:12198.
          http://www.ncbi.nlm.nih.gov/pubmed/27416963
          微生物病研究所 「主な研究成果」 紹介ページへ

        7. Shimada M, Goshima T, Matsuo H, Johmura Y, Haruta M, Murata K, Tanaka H, Ikawa M, Nakanishi K, Nakanishi M.
          Essential role of autoactivation circuitry on Aurora B-mediated H2AX-pS121 in mitosis.
          Nat Commun. 2016 Jul 8;7:12059.
          http://www.ncbi.nlm.nih.gov/pubmed/27389782

        8. Miyata H, Castaneda JM, Fujihara Y, Yu Z, Archambeault DR, Isotani A, Kiyozumi D, Kriseman ML, Mashiko D, Matsumura T, Matzuk RM, Mori M, Noda T, Oji A, Okabe M, Prunskaite-Hyyrylainen R, Ramirez-Solis R, Satouh Y, Zhang Q, Ikawa M, Matzuk MM.
          Genome engineering uncovers 54 evolutionarily conserved and testis-enriched genes that are not required for male fertility in mice.
          Proc Natl Acad Sci U S A. 2016 Jul 12;113(28):7704-10.
          http://www.ncbi.nlm.nih.gov/pubmed/27357688
          微生物病研究所 「主な研究成果」 紹介ページへ

        9. Kanemori Y, Koga Y, Sudo M, Kang W, Kashiwabara SI, Ikawa M, Hasuwa H, Nagashima K, Ishikawa Y, Ogonuki N, Ogura A, Baba T.
          Biogenesis of sperm acrosome is regulated by pre-mRNA alternative splicing of Acrbp in the mouse.
          Proc Natl Acad Sci U S A. 2016 Jun 28;113(26):E3696-705.
          http://www.ncbi.nlm.nih.gov/pubmed/27303034
          外部紹介ページへ

        10. Yamazaki D, Funato Y, Miyata H, Ikawa M, Miki H.
          Complementary role of CNNM2 in sperm motility and Ca2+ influx during capacitation.
          Biochem Biophys Res Commun. 2016 Jun 3;474(3):441-6.
          http://www.ncbi.nlm.nih.gov/pubmed/27150626

        11. Shimbo M, Kudo T, Hamada M, Jeon H, Imamura Y, Asano K, Okada R, Tsunakawa Y, Mizuno S, Yagami KI, Ishikawa C, Li H, Shiga T, Ishida J, Hamada J, Murata K, Ishimaru T, Hashimoto M, Fukamizu A, Yamane M, Ikawa M, Morita H, Shinohara M, Asahara H, Akiyama T, Akiyama N, Sasanuma H, Yoshida N, Zhou R, Wang YY, Ito T, Kokubu Y, Noguchi TK, Ishimine H, Kurisaki A, Shiba D, Mizuno H, Shirakawa M, Ito N, Takeda S, Takahashi S.
          Ground-based assessment of JAXA mouse habitat cage unit by mouse phenotypic studies.
          Exp Anim. 2016 May 20;65(2):175-87.
          http://www.ncbi.nlm.nih.gov/pubmed/26822934

        12. Yamazaki D, Miyata H, Funato Y, Fujihara Y, Ikawa M, Miki H.
          The Mg2+ transporter CNNM4 regulates sperm Ca2+ homeostasis and it is essential for reproduction.
          J Cell Sci. 2016 May 1;129(9):1940-9.
          http://www.ncbi.nlm.nih.gov/pubmed/27006114

        13. Kai T, Tsukamoto Y, Hijiya N, Tokunaga A, Nakada C, Uchida T, Daa T, Iha H, Takahashi M, Nomura T, Sato F, Mimata H, Ikawa M, Seto M, Matsuura K, Moriyama M.
          Kidney-specific knockout of Sav1 in the mouse promotes hyper-proliferation of renal tubular epithelium through suppression of the Hippo pathway.
          J Pathol. 2016 May;239(1):97-108.
          http://www.ncbi.nlm.nih.gov/pubmed/26913567

        14. Isotani A, Yamagata K, Okabe M, Ikawa M.
          Generation of Hprt-disrupted rat through mouse←rat ES chimeras.
          Sci Rep. 2016 Apr 11;6:24215.
          http://www.ncbi.nlm.nih.gov/pubmed/27062982

        15. Muro Y, Hasuwa H, Isotani A, Miyata H, Yamagata K, Ikawa M, Yanagimachi R, Okabe M.
          Behavior of Mouse Spermatozoa in the Female Reproductive Tract from Soon after Mating to the Beginning of Fertilization.
          Biol Reprod. 2016 Apr;94(4):80.
          http://www.ncbi.nlm.nih.gov/pubmed/26962112

        16. Brukman NG, Miyata H, Torres P, Lombardo D, Caramelo JJ, Ikawa M, Da Ros VG, Cuasnicú PS.
          Fertilization defects in sperm from Cysteine-rich secretory protein 2 (Crisp2) knockout mice: Implications for fertility disorders.
          Mol Hum Reprod. 2016 Apr;22(4):240-51.
          http://www.ncbi.nlm.nih.gov/pubmed/26786179

        17. Muto M, Fujihara Y, Tobita T, Kiyozumi D, Ikawa M.
          Lentiviral Vector Mediated Complementation Restored Fetal Viability but Not Placental hyperplasia in Plac1-Deficient Mice.
          Biol Reprod. 2016 Jan;94(1):6.
          http://www.ncbi.nlm.nih.gov/pubmed/26586843

        2015

        1. Zimmerman KA, Xing D, Pallero MA, Lu A, Ikawa M, Black L, Hoyt KL, Kabarowski JH, Michalak M, Murphy-Ullrich JE.
          Calreticulin Regulates Neointima Formation and Collagen Deposition following Carotid Artery Ligation.
          J Vasc Res. 2015;52(5):306-20.
          http://www.ncbi.nlm.nih.gov/pubmed/26910059

        2. Gyobu S, Miyata H, Ikawa M, Yamazaki D, Takeshima H, Suzuki J, Nagata S.
          A role of TMEM16E carrying a scrambling domain in sperm motility.
          Mol Cell Biol. 2015 Dec 14;36(4):645-59.
          http://www.ncbi.nlm.nih.gov/pubmed/26667038

        3. Latos PA, Sienerth AR, Murray A, Senner CE, Muto M, Ikawa M, Oxley D, Burge S, Cox BJ, Hemberger M.
          Elf5-centered transcription factor hub controls trophoblast stem cell self-renewal and differentiation through stoichiometry-sensitive shifts in target gene networks.
          Genes Dev. 2015 Dec 1;29(23):2435-48.
          http://www.ncbi.nlm.nih.gov/pubmed/26584622

        4. Miyata H, Satouh Y, Mashiko D, Muto M, Nozawa K, Shiba K, Fujihara Y, Isotani A, Inaba K, Ikawa M.
          Sperm calcineurin inhibition prevents mouse fertility with implications for male contraceptive.
          Science. 2015 Oct 23;350(6259):442-5.
          http://www.ncbi.nlm.nih.gov/pubmed/26429887
          微生物病研究所 「主な研究成果」 紹介ページへ
          You may find Abstract and Full text provided by AAAS/Science .

        5. Young SA, Miyata H, Satouh Y, Kato H, Nozawa K, Isotani A, Aitken RJ, Baker MA, Ikawa M.
          CRISPR/Cas9-Mediated Rapid Generation of Multiple Mouse Lines Identified Ccdc63 as Essential for Spermiogenesis.
          Int J Mol Sci. 2015 Oct 16;16(10):24732-24750.
          http://www.ncbi.nlm.nih.gov/pubmed/26501274

        6. Yamaguchi M, Watanabe Y, Ohtani T, Uezumi A, Mikami N, Nakamura M, Sato T, Ikawa M, Hoshino M, Tsuchida K, Miyagoe-Suzuki Y, Tsujikawa K, Takeda S, Yamamoto H, Fukada S.
          Calcitonin Receptor Signaling Inhibits Muscle Stem Cells from Escaping the Quiescent State and the Niche.
          Cell Rep. 2015 Oct 13;13(2):302-14.
          http://www.ncbi.nlm.nih.gov/pubmed/26440893

        7. Satouh Y, Nozawa K, Ikawa M.
          Sperm Postacrosomal WW Domain-Binding Protein Is Not Required for Mouse Egg Activation.
          Biol Reprod. 2015 Oct;93(4):94.
          http://www.ncbi.nlm.nih.gov/pubmed/26377222

        8. Tokuhiro K, Satouh Y, Nozawa K, Isotani A, Fujihara Y, Hirashima Y, Matsumura H, Takumi K, Miyano T, Okabe M, Benham AM, Ikawa M.
          Calreticulin is required for development of the cumulus oocyte complex and female fertility.
          Sci Rep. 2015 Sep 21;5:14254.
          http://www.ncbi.nlm.nih.gov/pubmed/26388295

        9. Ono R, Ishii M, Fujihara Y, Kitazawa M, Usami T, Kaneko-Ishino T, Kanno J, Ikawa M, Ishino F.
          Double strand break repair by capture of retrotransposon sequences and reverse-transcribed spliced mRNA sequences in mouse zygotes.
          Sci Rep. 2015 Jul 28;5:12281.
          http://www.ncbi.nlm.nih.gov/pubmed/26216318

        10. Kidoya H, Naito H, Muramatsu F, Yamakawa D, Jia W, Ikawa M, Sonobe T, Tsuchimochi H, Shirai M, Adams RH, Fukamizu A, Takakura N.
          APJ Regulates Parallel Alignment of Arteries and Veins in the Skin.
          Dev Cell. 2015 May 4;33(3):247-59.
          http://www.ncbi.nlm.nih.gov/pubmed/25920569

        11. Okuda H, Kiuchi H, Takao T, Miyagawa Y, Tsujimura A, Nonomura N, Miyata H, Okabe M, Ikawa M, Kawakami Y, Goshima N, Wada M, Tanaka H.
          A novel transcriptional factor nkapl is a germ cell-specific suppressor of notch signaling and is indispensable for spermatogenesis.
          PLoS One. 2015 Apr 14;10(4):e0124293.
          http://www.ncbi.nlm.nih.gov/pubmed/25875095

        12. Akıncılar SC, Low KC, Liu CY, Yan TD, Oji A, Ikawa M, Li S, Tergaonkar V.
          Quantitative assessment of telomerase components in cancer cell lines.
          FEBS Lett. 2015 Apr 13;589(9):974-84.
          http://www.ncbi.nlm.nih.gov/pubmed/25749370

        13. Ohtsuka M, Miura H, Mochida K, Hirose M, Hasegawa A, Ogura A, Mizutani R, Kimura M, Isotani A, Ikawa M, Sato M, Gurumurthy CB.
          One-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT).
          BMC Genomics. 2015 Apr 9;16:274.
          http://www.ncbi.nlm.nih.gov/pubmed/25887549

        14. Honda A, Hirose M, Sankai T, Yasmin L, Yuzawa K, Honsho K, Izu H, Iguchi A, Ikawa M, Ogura A.
          Single-step generation of rabbits carrying a targeted allele of the tyrosinase gene using CRISPR/Cas9.
          Exp Anim. 2015 Feb 5;64(1):31-7.
          http://www.ncbi.nlm.nih.gov/pubmed/25195632

        15. Kimura T, Kaga Y, Sekita Y, Fujikawa K, Nakatani T, Odamoto M, Funaki S, Ikawa M, Abe K, Nakano T.
          Pluripotent stem cells derived from mouse primordial germ cells by small molecule compounds.
          Stem Cells. 2015 Jan;33(1):45-55.
          http://www.ncbi.nlm.nih.gov/pubmed/25186651

        2014

        1. Qiu B, Shi X, Wong ET, Lim J, Bezzi M, Low D, Zhou Q, Akıncılar SC, Lakshmanan M, Swa HL, Tham JM, Gunaratne J, Cheng KK, Hong W, Lam KS, Ikawa M, Guccione E, Xu A, Han W, Tergaonkar V.
          NUCKS Is a Positive Transcriptional Regulator of Insulin Signaling.
          Cell Rep. 2014 Jun 26;7(6):1876-86.
          http://www.ncbi.nlm.nih.gov/pubmed/24931609

        2. Chenga j, Morisaki H, Sugimoto N, Dohi A, Shintani T, Kimura E, Toyama K, Ikawa M, Okabe M, Higuchi I, Matsuo S, Kawai Y, Hisatome I, Sugama T, Holmes E. W. , Morisaki T.
          Effect of isolated AMP deaminase deficiency on skeletal muscle function.
          Molecular Genetics and Metabolism Reports 2014;1:51-9
          http://www.sciencedirect.com/science/article/pii/S2214426913000098

        3. Mizuno S, Dinh TT, Kato K, Mizuno-Iijima S, Tanimoto Y, Daitoku Y, Hoshino Y, Ikawa M, Takahashi S, Sugiyama F, Yagami KI.
          Simple generation of albino C57BL/6J mice with G291T mutation in the tyrosinase gene by the CRISPR/Cas9 system.
          Mamm Genome. 2014 Aug;25(7-8):327-34.
          http://www.ncbi.nlm.nih.gov/pubmed/24879364

        4. Takarada-Iemata M, Kezuka D, Takeichi T, Ikawa M, Hattori T, Kitao Y, Hori O.
          Deletion of N-myc downstream-regulated gene 2 attenuates reactive astrogliosis and inflammatory response in a mouse model of cortical stab injury.
          J Neurochem. 2014 Aug;130(3):374-87.
          http://www.ncbi.nlm.nih.gov/pubmed/24697507

        5. Fujihara Y, Okabe M, Ikawa M.
          GPI-Anchored Protein Complex, LY6K/TEX101, Is Required for Sperm Migration into the Oviduct and Male Fertility in Mice.
          Biol Reprod. 2014 Mar 20;90(3):60.
          http://www.ncbi.nlm.nih.gov/pubmed/24501175

        6. Azuma K, Tsukui T, Ikeda K, Shiba S, Nakagawa K, Okano T, Urano T, Horie-Inoue K, Ouchi Y, Ikawa M, Inoue S.
          Liver-Specific γ-Glutamyl Carboxylase-Deficient Mice Display Bleeding Diathesis and Short Life Span.
          PLoS One. 2014 Feb 10;9(2):e88643.
          http://www.ncbi.nlm.nih.gov/pubmed/24520408

        7. Mashiko D, Young SA, Muto M, Kato H, Nozawa K, Ogawa M, Noda T, Kim YJ, Satouh Y, Fujihara Y, Ikawa M.
          Feasibility for a large scale mouse mutagenesis by injecting CRISPR/Cas plasmid into zygotes.
          Dev Growth Differ. 2014 Jan;56(1):122-9.
          http://www.ncbi.nlm.nih.gov/pubmed/24372541

        2013

        1. Mashiko D, Fujihara Y, Satouh Y, Miyata H, Isotani A, Ikawa M.
          Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA.
          Sci Rep. 2013 Nov 27;3:3355.
          http://www.ncbi.nlm.nih.gov/pubmed/24284873

        2. Ochiai N, Nishizuka M, Miyamoto T, Miyoshi I, Ikawa M, Osada S, Imagawa M.
          Targeted disruption of fad24, a regulator of adipogenesis, causes pre-implantation embryonic lethality due to the growth defect at the blastocyst stage.
          Biochem Biophys Res Commun. 2013 Aug 23;438(2):301-5.
          http://www.ncbi.nlm.nih.gov/pubmed/23886952

        3. Suzuki N, Kunishima S, Ikejiri M, Maruyama S, Sone M, Takagi A, Ikawa M, Okabe M, Kojima T, Saito H, Naoe T, Matsushita T.
          Establishment of mouse model of MYH9 disorders: heterozygous R702C mutation provokes macrothrombocytopenia with leukocyte inclusion bodies, renal glomerulosclerosis and hearing disability.
          PLoS One. 2013 Aug 20;8(8):e71187.
          http://www.ncbi.nlm.nih.gov/pubmed/23976996

        4. Tashiro-Yamaji J, Maeda S, Ikawa M, Okabe M, Kubota T, Yoshida R.
          Macrophage MHC and T-cell receptors essential for rejection of allografted skin and lymphoma.
          Transplantation. 2013 Aug 15;96(3):251-7.
          http://www.ncbi.nlm.nih.gov/pubmed/23836286

        5. Inoue N, Hamada D, Kamikubo H, Hirata K, Kataoka M, Yamamoto M, Ikawa M, Okabe M and Hagihara Y.
          Molecular dissection of IZUMO1, a sperm protein essential for sperm-egg fusion.
          Development.2013 Aug;140(15):3221-9.
          http://www.ncbi.nlm.nih.gov/pubmed/23824580

        6. Hasuwa H, Ueda J, Ikawa M, Okabe M.
          MiR-200b and miR-429 Function in Mouse Ovulation and Are Essential for Female Fertility.
          Science. 2013 Jul 5;341(6141):71-3.
          http://www.ncbi.nlm.nih.gov/pubmed/23765281
          PDF file
          微生物病研究所 「主な研究成果」 紹介ページへ

        7. Naito Y, Yabuta N, Sato J, Ohno S, Sakata M, Kasama T, Ikawa M, Nojima H.
          Recruitment of cyclin G2 to promyelocytic leukemia nuclear bodies promotes dephosphorylation of γH2AX following treatment with ionizing radiation.
          Cell Cycle. 2013 Jun 1;12(11):1773-84.
          http://www.ncbi.nlm.nih.gov/pubmed/23656780

        8. Fujihara Y, Tokuhiro K, Muro Y, Kondoh G, Araki Y, Ikawa M, Okabe M.
          Expression of TEX101, regulated by ACE, is essential for the production of fertile mouse spermatozoa.
          Proc Natl Acad Sci U S A. 2013 May 14;110(20):8111-6.
          http://www.ncbi.nlm.nih.gov/pubmed/23633567

        9. Nojima S, Toyofuku T, Kamao H, Ishigami C, Kaneko J, Okuno T, Takamatsu H, Ito D, Kang S, Kimura T, Yoshida Y, Morimoto K, Maeda Y, Ogata A, Ikawa M, Morii E, Aozasa K, Takagi J, Takahashi M, Kumanogoh A.
          A point mutation in Semaphorin 4A associates with defective endosomal sorting and causes retinal degeneration.
          Nat Commun. 2013;4:1406.
          http://www.ncbi.nlm.nih.gov/pubmed/23360997

        10. Fujihara Y, Kaseda K, Inoue N, Ikawa M, Okabe M.
          Production of mouse pups from germline transmission-failed knockout chimeras.
          Transgenic Res. 2013 Feb;22(1):195-200.
          http://www.ncbi.nlm.nih.gov/pubmed/22826106

        11. Yabuta N, Mukai S, Okamoto A, Okuzaki D, Suzuki H, Torigata K, Yoshida K, Okada N, Miura D, Ito A, Ikawa M, Okabe M, Nojima H.
          N-terminal truncation of Lats1 causes abnormal cell growth control and chromosomal instability.
          J Cell Sci. 2013 Jan 15;126(Pt 2):508-20.
          http://www.ncbi.nlm.nih.gov/pubmed/23230145

        12. Wang J, Shiratori I, Uehori J, Ikawa M, Arase H.
          Neutrophil infiltration during inflammation is regulated by PILRα via modulation of integrin activation.
          Nat Immunol. 2013 Jan;14(1):34-40.
          http://www.ncbi.nlm.nih.gov/pubmed/23142774

        2012

        1. Endo Y, Takahashi M, Iwaki D, Ishida Y, Nakazawa N, Kodama T, Matsuzaka T, Kanno K, Liu Y, Tsuchiya K, Kawamura I, Ikawa M, Waguri S, Wada I, Matsushita M, Schwaeble WJ, Fujita T.
          Mice deficient in ficolin, a lectin complement pathway recognition molecule, are susceptible to Streptococcus pneumoniae infection.
          J Immunol. 2012 Dec 15;189(12):5860-6.
          http://www.ncbi.nlm.nih.gov/pubmed/23150716

        2. Cheng J, Morisaki H, Toyama K, Ikawa M, Okabe M, Morisaki T.
          AMPD3-deficient mice exhibit increased erythrocyte ATP levels but anemia not improved due to PK deficiency.
          Genes Cells. 2012 Nov;17(11):913-22.
          http://www.ncbi.nlm.nih.gov/pubmed/23078545

        3. Satouh Y, Inoue N, Ikawa M, Okabe M.
          Visualization of the moment of mouse sperm-egg fusion and dynamic localization of IZUMO1.
          J Cell Sci. 2012 Nov 1;125(Pt 21):4985-90.
          http://www.ncbi.nlm.nih.gov/pubmed/22946049

        4. Fujihara Y, Satouh Y, Inoue N, Isotani A, Ikawa M, Okabe M.
          SPACA1-deficient male mice are infertile with abnormally shaped sperm heads reminiscent of globozoospermia.
          Development. 2012 Oct;139(19):3583-9
          http://www.ncbi.nlm.nih.gov/pubmed/22949614

        5. Inoue N, Nishikawa T, Ikawa M, Okabe M.
          Tetraspanin-interacting protein IGSF8 is dispensable for mouse fertility.
          Fertil Steril. 2012 Aug;98(2):465-70.
          http://www.ncbi.nlm.nih.gov/pubmed/22609062

        6. Tarutani M, Nakajima K, Uchida Y, Takaishi M, Goto-Inoue N, Ikawa M, Setou M, Kinoshita T, Elias PM, Sano S, Maeda Y.
          GPHR-dependent functions of the Golgi apparatus are essential for the formation of lamellar granules and the skin barrier.
          J Invest Dermatol. 2012 Aug;132(8):2019-25.
          http://www.ncbi.nlm.nih.gov/pubmed/22572823

        7. Yamaguchi R, Fujihara Y, Ikawa M, Okabe M.
          Mice expressing aberrant sperm-specific protein PMIS2 produce normal-looking but fertilization-incompetent spermatozoa.
          Mol Biol Cell. 2012 Jul;23(14):2671-9.
          http://www.ncbi.nlm.nih.gov/pubmed/22621904

        8. Masuda T, Itoh K, Higashitsuji H, Higashitsuji H, Nakazawa N, Sakurai T, Liu Y, Tokuchi H, Fujita T, Zhao Y, Nishiyama H, Tanaka T, Fukumoto M, Ikawa M, Okabe M, Fujita J.
          Cold-inducible RNA-binding protein (Cirp) interacts with Dyrk1b/Mirk and promotes proliferation of immature male germ cells in mice.
          Proc Natl Acad Sci U S A. 2012 Jul 3;109(27):10885-90.
          http://www.ncbi.nlm.nih.gov/pubmed/22711815

        9. Tokuhiro K, Ikawa M, Benham AM, Okabe M.
          Protein disulfide isomerase homolog PDILT is required for quality control of sperm membrane protein ADAM3 and male fertility.
          Proc Natl Acad Sci U S A. 2012 Mar 6;109(10):3850-5.
          http://www.ncbi.nlm.nih.gov/pubmed/22357757

        10. Ito D, Yagi T, Ikawa M, Suzuki N.
          Characterization of inclusion bodies with cytoprotective properties formed by seipinopathy-linked mutant seipin.
          Hum Mol Genet. 2012 Feb 1;21(3):635-46.
          http://www.ncbi.nlm.nih.gov/pubmed/22045697

        11. Toyama K, Morisaki H, Cheng J, Kawachi H, Shimizu F, Ikawa M, Okabe M, Morisaki T.
          Proteinuria in AMPD2-deficient mice.
          Genes Cells. 2012 Jan;17(1):28-38.
          http://www.ncbi.nlm.nih.gov/pubmed/22212473

        2011

        1. Inoue N, Satouh Y, Ikawa M, Okabe M, Yanagimachi R.
          Acrosome-reacted mouse spermatozoa recovered from the perivitelline space can fertilize other eggs.
          Proc Natl Acad Sci U S A. 2011 Dec 13;108(50):20008-11.
          http://www.ncbi.nlm.nih.gov/pubmed/22084105

        2. Hayashi T, Nozaki Y, Nishizuka M, Ikawa M, Osada S, Imagawa M.
          Factor for adipocyte differentiation 158 gene disruption prevents the body weight gain and insulin resistance induced by a high-fat diet.
          Biol Pharm Bull. 2011;34(8):1257-63.
          http://www.ncbi.nlm.nih.gov/pubmed/21804215

        3. Mitsutake S, Zama K, Yokota H, Yoshida T, Tanaka M, Mitsui M, Ikawa M, Okabe M, Tanaka Y, Yamashita T, Takemoto H, Okazaki T, Watanabe K, Igarashi Y.
          Dynamic modification of sphingomyelin in lipid microdomains controls development of obesity, fatty liver, and type 2 diabetes.
          J Biol Chem. 2011; 286(32):28544-55.
          http://www.ncbi.nlm.nih.gov/pubmed/21669879

        4. Kanatsu-Shinohara M, Kato-Itoh M, Ikawa M, Takehashi M, Sanbo M, Morioka Y, Tanaka T, Morimoto H, Hirabayashi M, Shinohara T.
          Homologous recombination in rat germline stem cells.
          Biol Reprod. 2011 Jul;85(1):208-17.
          http://www.ncbi.nlm.nih.gov/pubmed/21471297

        5. Suga T, Kimura E, Morioka Y, Ikawa M, Li S, Uchino K, Uchida Y, Yamashita S, Maeda Y, Chamberlain JS, Uchino M.
          Muscle fiber type-predominant promoter activity in lentiviral-mediated transgenic mouse.
          PLoS One. 2011 Mar 18;6(3):e16908.
          http://www.ncbi.nlm.nih.gov/pubmed/21445245

        6. Nagai M, Moriyama T, Mehmood R, Tokuhiro K, Ikawa M, Okabe M, Tanaka H, Yoneda Y.
          Mice lacking Ran binding protein 1 are viable and show male infertility.
          FEBS Lett. 2011; 585(5):791-6.
          http://www.ncbi.nlm.nih.gov/pubmed/21310149

        7. Moriyama T, Nagai M, Oka M, Ikawa M, Okabe M, Yoneda Y.
          Targeted disruption of one of the importin α family members leads to female functional incompetence in delivery.
          FEBS J. 2011; 278(9):1561-72.
          http://www.ncbi.nlm.nih.gov/pubmed/21371262

        8. Isotani A, Hatayama H, Kaseda K, Ikawa M, Okabe M.
          Formation of a thymus from rat ES cells in xenogeneic nude mouse?rat ES chimeras.
          Genes Cells. 2011; 16:397-405.
          http://www.ncbi.nlm.nih.gov/pubmed/21401810

        9. Nakagawa Y, Takamatsu H, Okuno T, Kang S, Nojima S, Kimura T, Kataoka TR, Ikawa M, Toyofuku T, Katayama I, Kumanogoh A.
          Identification of semaphorin 4B as a negative regulator of basophil-mediated immune responses.
          J Immunol. 2011 Mar 1;186(5):2881-8.
          http://www.ncbi.nlm.nih.gov/pubmed/21270411

        10. Yano M, Watanabe K, Yamamoto T, Ikeda K, Senokuchi T, Lu M, Kadomatsu T, Tsukano H, Ikawa M, Okabe M, Yamaoka S, Okazaki T, Umehara H, Gotoh T, Song WJ, Node K, Taguchi R, Yamagata K, Oike Y.
          Mitochondrial dysfunction and increased reactive oxygen species impair insulin secretion in sphingomyelin synthase 1-null mice.
          J Biol Chem. 2011; 286(5):3992-4002.
          http://www.ncbi.nlm.nih.gov/pubmed/21115496

        11. Kumasawa K, Ikawa M, Kidoya H, Hasuwa H, Saito-Fujita T, Morioka Y, Takakura N, Kimura T, Okabe M.
          Pravastatin induces placental growth factor (PGF) and ameliorates preeclampsia in a mouse model.
          Proc Natl Acad Sci U S A 2011; 108:1451-1455.
          http://www.ncbi.nlm.nih.gov/pubmed/21187414
          微生物病研究所 「主な研究成果」 紹介ページへ

        12. Ikawa M, Tokuhiro K, Yamaguchi R, Benham AM, Tamura T, Wada I, Satouh Y, Inoue N, Okabe M.
          Calsperin is a testis-specific chaperone required for sperm fertility.
          J Biol Chem. 2011; 286(7):5639-46. http://www.ncbi.nlm.nih.gov/pubmed/21131354

        2010

        1. Inoue N, Kasahara T, Ikawa M, Okabe M.
          Identification and disruption of sperm-specific angiotensin converting enzyme-3 (ACE3) in mouse.
          PLoS ONE 2010; 5:e10301.
          http://www.ncbi.nlm.nih.gov/pubmed/20421979

        2. Ichise T, Adachi S, Ohishi M, Ikawa M, Okabe M, Iwamoto R, Mekada E.
          Humanized gene replacement in mice reveals the contribution of cancer stroma-derived HB-EGF to tumor growth.
          Cell Struct Funct 2010; 35:3-13.
          http://www.ncbi.nlm.nih.gov/pubmed/20190463

        3. Hosoyamada M, Takiue Y, Morisaki H, Cheng J, Ikawa M, Okabe M, Morisaki T, Ichida K, Hosoya T, Shibasaki T.
          Establishment and analysis of SLC22A12 (URAT1) knockout mouse.
          Nucleosides Nucleotides Nucleic Acids 2010; 29:314-320.
          http://www.ncbi.nlm.nih.gov/pubmed/20544513

        4. Hasuwa H, Muro Y, Ikawa M, Kato N, Tsujimoto Y, Okabe M.
          Transgenic mouse sperm that have green acrosome and red mitochondria allow visualization of sperm and their acrosome reaction in vivo.

          Exp Anim 2010; 59:105-107.
          http://www.ncbi.nlm.nih.gov/pubmed/20224175

        5. Fujihara Y, Murakami M, Inoue N, Satouh Y, Kaseda K, Ikawa M, Okabe M.
          Sperm equatorial segment protein 1, SPESP1, is required for fully fertile sperm in mouse.
          J Cell Sci 2010; 123:1531-1536.
          http://www.ncbi.nlm.nih.gov/pubmed/20375058

        6. Shibukawa Y, Yamazaki N, Kumasawa K, Daimon E, Tajiri M, Okada Y, Ikawa M, Wada Y.
          Calponin 3 regulates actin cytoskeleton rearrangement in trophoblastic cell fusion.
          Mol Biol Cell. 2010; 21(22):3973-84.
          http://www.ncbi.nlm.nih.gov/pubmed/20861310

        2009

        1. Yamaguchi R, Muro Y, Isotani A, Tokuhiro K, Takumi K, Adham I, Ikawa M, Okabe M.
          Disruption of ADAM3 impairs the migration of sperm into oviduct in mouse.
          Biol Reprod 2009; 81:142-146.
          http://www.ncbi.nlm.nih.gov/pubmed/19339711

        2. Saito A, Hino S, Murakami T, Kanemoto S, Kondo S, Saitoh M, Nishimura R, Yoneda T, Furuichi T, Ikegawa S, Ikawa M, Okabe M, et al.
          Regulation of endoplasmic reticulum stress response by a BBF2H7-mediated Sec23a pathway is essential for chondrogenesis.
          Nat Cell Biol 2009; 11:1197-1204.
          http://www.ncbi.nlm.nih.gov/pubmed/19767744

        3. Okada Y, Ueshin Y, Hasuwa H, Takumi K, Okabe M, Ikawa M.
          Targeted gene modification in mouse ES cells using integrase-defective lentiviral vectors.
          Genesis 2009; 47:217-223.
          http://www.ncbi.nlm.nih.gov/pubmed/19208434

        4. Ohta H, Ohinata Y, Ikawa M, Morioka Y, Sakaide Y, Saitou M, Kanagawa O, Wakayama T.
          Male germline and embryonic stem cell lines from NOD mice: efficient derivation of GS cells from a nonpermissive strain for ES cell derivation.
          Biol Reprod. 2009; 81(6):1147-53.
          http://www.ncbi.nlm.nih.gov/pubmed/19726737

        5. Nishizuka M, Kishimoto K, Kato A, Ikawa M, Okabe M, Sato R, Niida H, Nakanishi M, Osada S, Imagawa M.
          Disruption of the novel gene fad104 causes rapid postnatal death and attenuation of cell proliferation, adhesion, spreading and migration.
          Exp Cell Res 2009; 315:809-819.
          http://www.ncbi.nlm.nih.gov/pubmed/19138685

        6. Nimura K, Ura K, Shiratori H, Ikawa M, Okabe M, Schwartz RJ, Kaneda Y.
          A histone H3 lysine 36 trimethyltransferase links Nkx2-5 to Wolf-Hirschhorn syndrome.
          Nature 2009; 460:287-291.
          http://www.ncbi.nlm.nih.gov/pubmed/19483677

        7. Nagaoka K, Aoki F, Hayashi M, Muroi Y, Sakurai T, Itoh K, Ikawa M, Okabe M, Imakawa K, Sakai S.
          L-amino acid oxidase plays a crucial role in host defense in the mammary glands.
          FASEB J 2009; 23:2514-2520.
          http://www.ncbi.nlm.nih.gov/pubmed/19276171

        8. Murakami T, Saito A, Hino S, Kondo S, Kanemoto S, Chihara K, Sekiya H, Tsumagari K, Ochiai K, Yoshinaga K, Saitoh M, Nishimura R, et al.
          Signalling mediated by the endoplasmic reticulum stress transducer OASIS is involved in bone formation.
          Nat Cell Biol 2009; 11:1205-1211.
          http://www.ncbi.nlm.nih.gov/pubmed/19767743

        9. Morioka Y, Isotani A, Oshima RG, Okabe M, Ikawa M.
          Placenta-specific gene activation and inactivation using integrase-defective lentiviral vectors with the Cre/LoxP system.
          Genesis 2009; 47:793-798.
          http://www.ncbi.nlm.nih.gov/pubmed/19830817

        10. Kawasaki T, Saito K, Mitsui K, Ikawa M, Yamashita M, Taniguchi Y, Takeda S, Mitani K, Sakai N.
          Introduction of a foreign gene into zebrafish and medaka cells using adenoviral vectors.
          Genesis 2009; 6(3):253-8.
          http://www.ncbi.nlm.nih.gov/pubmed/19715489

        11. Iuchi Y, Okada F, Tsunoda S, Kibe N, Shirasawa N, Ikawa M, Okabe M, Ikeda Y, Fujii J.
          Peroxiredoxin 4 knockout results in elevated spermatogenic cell death via oxidative stress.
          Biochem J 2009; 419:149-158.
          http://www.ncbi.nlm.nih.gov/pubmed/19105792

        2008

        1. Youn DY, Lee DH, Lim MH, Yoon JS, Lim JH, Jung SE, Yeum CE, Park CW, Youn HJ, Lee JS, Lee SB, Ikawa M, et al.
          Bis deficiency results in early lethality with metabolic deterioration and involution of spleen and thymus.
          Am J Physiol Endocrinol Metab 2008; 295:E1349-1357.
          http://www.ncbi.nlm.nih.gov/pubmed/18840758

        2. Yamaguchi R, Yamagata K, Hasuwa H, Inano E, Ikawa M, Okabe M.
          Cd52, known as a major maturation-associated sperm membrane antigen secreted from the epididymis, is not required for fertilization in the mouse.
          Genes Cells 2008; 13:851-861.
          http://www.ncbi.nlm.nih.gov/pubmed/18782223

        3. Shinzawa K, Sumi H, Ikawa M, Matsuoka Y, Okabe M, Sakoda S, Tsujimoto Y.
          Neuroaxonal dystrophy caused by group VIA phospholipase A2 deficiency in mice: a model of human neurodegenerative disease.
          J Neurosci 2008; 28:2212-2220.
          http://www.ncbi.nlm.nih.gov/pubmed/18305254

        4. Okiyoneda T, Niibori A, Harada K, Kohno T, Michalak M, Duszyk M, Wada I, Ikawa M, Shuto T, Suico MA, Kai H.
          Role of calnexin in the ER quality control and productive folding of CFTR; differential effect of calnexin knockout on wild-type and DeltaF508 CFTR.
          Biochim Biophys Acta. 2008; 1783(9):1585-94.
          http://www.ncbi.nlm.nih.gov/pubmed/18457676

        5. Kuramochi-Miyagawa S, Watanabe T, Gotoh K, Totoki Y, Toyoda A, Ikawa M, Asada N, Kojima K, Yamaguchi Y, Ijiri TW, Hata K, Li E, et al.
          DNA methylation of retrotransposon genes is regulated by Piwi family members MILI and MIWI2 in murine fetal testes.
          Genes Dev 2008; 22:908-917.
          http://www.ncbi.nlm.nih.gov/pubmed/18381894

        6. Kanatsu-Shinohara M, Lee J, Inoue K, Ogonuki N, Miki H, Toyokuni S, Ikawa M, Nakamura T, Ogura A, Shinohara T.
          Pluripotency of a single spermatogonial stem cell in mice.
          Biol Reprod. 2008; 78(4):681-7.
          http://www.ncbi.nlm.nih.gov/pubmed/18199882

        7. Ito T, Kimura Y, Uozumi Y, Takai M, Muraoka S, Matsuda T, Ueki K, Yoshiyama M, Ikawa M, Okabe M, Schaffer SW, Fujio Y, et al.
          Taurine depletion caused by knocking out the taurine transporter gene leads to cardiomyopathy with cardiac atrophy.
          J Mol Cell Cardiol 2008; 44:927-937.
          http://www.ncbi.nlm.nih.gov/pubmed/18407290

        8. Inoue N, Ikawa M, Okabe M.
          Putative sperm fusion protein IZUMO and the role of N-glycosylation.
          Biochem Biophys Res Commun 2008; 377:910-914.
          http://www.ncbi.nlm.nih.gov/pubmed/18952059

        2007

        1. Yabuta N, Okada N, Ito A, Hosomi T, Nishihara S, Sasayama Y, Fujimori A, Okuzaki D, Zhao H, Ikawa M, Okabe M, Nojima H.
          Lats2 is an essential mitotic regulator required for the coordination of cell division.
          J Biol Chem 2007; 282:19259-19271.
          http://www.ncbi.nlm.nih.gov/pubmed/17478426

        2. Umehara H, Kimura T, Ohtsuka S, Nakamura T, Kitajima K, Ikawa M, Okabe M, Niwa H, Nakano T.
          Efficient derivation of embryonic stem cells by inhibition of glycogen synthase kinase-3.
          Stem Cells 2007; 25:2705-2711.
          http://www.ncbi.nlm.nih.gov/pubmed/17641246

        3. Ueda Y, Yamaguchi R, Ikawa M, Okabe M, Morii E, Maeda Y, Kinoshita T.
          PGAP1 knock-out mice show otocephaly and male infertility.
          J Biol Chem 2007; 282:30373-30380.
          http://www.ncbi.nlm.nih.gov/pubmed/17711852

        4. Tsujikawa K, Yayama K, Hayashi T, Matsushita H, Yamaguchi T, Shigeno T, Ogitani Y, Hirayama M, Kato T, Fukada S, Takatori S, Kawasaki H, et al.
          Hypertension and dysregulated proinflammatory cytokine production in receptor activity-modifying protein 1-deficient mice.
          Proc Natl Acad Sci U S A 2007; 104:16702-16707.
          http://www.ncbi.nlm.nih.gov/pubmed/17923674

        5. Okada Y, Ueshin Y, Isotani A, Saito-Fujita T, Nakashima H, Kimura K, Mizoguchi A, Oh-Hora M, Mori Y, Ogata M, Oshima RG, Okabe M, et al.
          Complementation of placental defects and embryonic lethality by trophoblast-specific lentiviral gene transfer.
          Nat Biotechnol 2007; 25:233-237.
          http://www.ncbi.nlm.nih.gov/pubmed/17220877

        6. Nakamura T, Arai Y, Umehara H, Masuhara M, Kimura T, Taniguchi H, Sekimoto T, Ikawa M, Yoneda Y, Okabe M, Tanaka S, Shiota K, et al.
          PGC7/Stella protects against DNA demethylation in early embryogenesis.
          Nat Cell Biol 2007; 9:64-71.
          http://www.ncbi.nlm.nih.gov/pubmed/17143267

        7. Kobayakawa K, Kobayakawa R, Matsumoto H, Oka Y, Imai T, Ikawa M, Okabe M, Ikeda T, Itohara S, Kikusui T, Mori K, Sakano H.
          Innate versus learned odour processing in the mouse olfactory bulb.
          Nature 2007; 450:503-508.
          http://www.ncbi.nlm.nih.gov/pubmed/17989651

        8. Akazawa T, Ebihara T, Okuno M, Okuda Y, Shingai M, Tsujimura K, Takahashi T, Ikawa M, Okabe M, Inoue N, Okamoto-Tanaka M, Ishizaki H, et al.
          Antitumor NK activation induced by the Toll-like receptor 3-TICAM-1 (TRIF) pathway in myeloid dendritic cells.
          Proc Natl Acad Sci U S A 2007; 104:252-257.
          http://www.ncbi.nlm.nih.gov/pubmed/17190817

        2006 and before

        1. Zhao H, Ito A, Kimura SH, Yabuta N, Sakai N, Ikawa M, Okabe M, Matsuzawa Y, Yamashita S, Nojima H.
          RECS1 deficiency in mice induces susceptibility to cystic medial degeneration.
          Genes Genet Syst 2006; 81:41-50.
          http://www.ncbi.nlm.nih.gov/pubmed/16607040

        2. Yamaguchi R, Yamagata K, Ikawa M, Moss SB, Okabe M.
          Aberrant Distribution of ADAM3 in Sperm from Both Angiotensin-Converting Enzyme (Ace)- and Calmegin (Clgn)-Deficient Mice.
          Biol Reprod 2006; 75:760-766.
          http://www.ncbi.nlm.nih.gov/pubmed/16870943

        3. Tanaka N, Ikawa M, Mata NL, Verma IM.
          Choroidal neovascularization in transgenic mice expressing prokineticin 1: an animal model for age-related macular degeneration.
          Mol Ther. 2006; 13(3):609-16.
          http://www.ncbi.nlm.nih.gov/pubmed/16263331

        4. Takegahara N, Takamatsu H, Toyofuku T, Tsujimura T, Okuno T, Yukawa K, Mizui M, Yamamoto M, Prasad DV, Suzuki K, Ishii M, Terai K, et al.
          Plexin-A1 and its interaction with DAP12 in immune responses and bone homeostasis.
          Nat Cell Biol 2006; 8:615-622.
          http://www.ncbi.nlm.nih.gov/pubmed/16715077

        5. Singer O, Tiscornia G, Ikawa M, Verma IM. Rapid generation of knockdown transgenic mice by silencing lentiviral vectors. Nat Protoc. 2006; 1(1):286-92. http://www.ncbi.nlm.nih.gov/pubmed/17406246

        6. Nagamori I, Yomogida K, Ikawa M, Okabe M, Yabuta N, Nojima H.
          The testes-specific bZip type transcription factor Tisp40 plays a role in ER stress responses and chromatin packaging during spermiogenesis.
          Genes Cells 2006; 11:1161-1171.
          http://www.ncbi.nlm.nih.gov/pubmed/16999736

        7. Kobayashi S, Isotani A, Mise N, Yamamoto M, Fujihara Y, Kaseda K, Nakanishi T, Ikawa M, Hamada H, Abe K, Okabe M.
          Comparison of gene expression in male and female mouse blastocysts revealed imprinting of the x-linked gene, rhox5/pem, at preimplantation stages.
          Curr Biol 2006; 16:166-172.
          http://www.ncbi.nlm.nih.gov/pubmed/16431368

        8. Kanatsu-Shinohara M, Ikawa M, Takehashi M, Ogonuki N, Miki H, Inoue K, Kazuki Y, Lee J, Toyokuni S, Oshimura M, Ogura A, Shinohara T.
          Production of knockout mice by random or targeted mutagenesis in spermatogonial stem cells.
          Proc Natl Acad Sci U S A. 2006; 103(21):8018-23.
          http://www.ncbi.nlm.nih.gov/pubmed/16679411

        9. Hori O, Miyazaki M, Tamatani T, Ozawa K, Takano K, Okabe M, Ikawa M, Hartmann E, Mai P, Stern DM, Kitao Y, Ogawa S.
          Deletion of SERP1/RAMP4, a component of the endoplasmic reticulum (ER) translocation sites, leads to ER stress.
          Mol Cell Biol 2006; 26:4257-4267.
          http://www.ncbi.nlm.nih.gov/pubmed/16705175

        10. Uchida S, Sohara E, Rai T, Ikawa M, Okabe M, Sasaki S.
          Impaired Urea Accumulation in the Inner Medulla of Mice Lacking the Urea Transporter UT-A2.
          Mol Cell Biol. 2005; 25:7357-7363.
          http://www.ncbi.nlm.nih.gov/pubmed/16055743

        11. Tergaonkar V, Correa RG, Ikawa M, Verma IM.
          Distinct roles of IkappaB proteins in regulating constitutive NF-kappaB activity.
          Nat Cell Biol. 2005; 7(9):921-3.
          http://www.ncbi.nlm.nih.gov/pubmed/16136188

        12. Inoue N, Ikawa M, Isotani A, Okabe M.
          The immunoglobulin superfamily protein Izumo is required for sperm to fuse with eggs.
          Nature. 2005; 434:234-238.
          http://www.ncbi.nlm.nih.gov/pubmed/15759005
          微生物病研究所 「主な研究成果」 紹介ページへ

        13. Hara-Chikuma M, Sohara E, Rai T, Ikawa M, Okabe M, Sasaki S, Uchida S, Verkman AS.
          Progressive adipocyte hypertrophy in aquaporin-7-deficient mice: adipocyte glycerol permeability as a novel regulator of fat accumulation.
          J Biol Chem. 2005; 280:15493-15496.
          http://www.ncbi.nlm.nih.gov/pubmed/15746100

        14. Shiomi N, Kito S, Oyama M, Matsunaga T, Harada YN, Ikawa M, Okabe M, Shiomi T.
          Identification of the XPG region that causes the onset of Cockayne syndrome by using Xpg mutant mice generated by the cDNA-mediated knock-in method.
          Mol Cell Biol 2004; 24:3712-3719.
          http://www.ncbi.nlm.nih.gov/pubmed/15082767

        15. Nakanishi T, Isotani A, Yamaguchi R, Ikawa M, Baba T, Suarez SS, Okabe M.
          Selective passage through the uterotubal junction of sperm from a mixed population produced by chimeras of calmegin-knockout and wild-type male mice.
          Biol Reprod 2004; 71:959-965.
          http://www.ncbi.nlm.nih.gov/pubmed/15151931

        16. Mori T, Yuxing Z, Takaki H, Takeuchi M, Iseki K, Hagino S, Kitanaka J, Takemura M, Misawa H, Ikawa M, Okabe M, Wanaka A.
          The LIM homeobox gene, L3/Lhx8, is necessary for proper development of basal forebrain cholinergic neurons.
          Eur J Neurosci 2004; 19:3129-3141.
          http://www.ncbi.nlm.nih.gov/pubmed/15217369

        17. Matsumura H, Hasuwa H, Inoue N, Ikawa M, Okabe M.
          Lineage-specific cell disruption in living mice by Cre-mediated expression of diphtheria toxin A chain.
          Biochem Biophys Res Commun 2004; 321:275-279.
          http://www.ncbi.nlm.nih.gov/pubmed/15358172

        18. Kuramochi-Miyagawa S, Kimura T, Ijiri TW, Isobe T, Asada N, Fujita Y, Ikawa M, Iwai N, Okabe M, Deng W, Lin H, Matsuda Y, et al.
          Mili, a mammalian member of piwi family gene, is essential for spermatogenesis.
          Development 2004; 131:839-849.
          http://www.ncbi.nlm.nih.gov/pubmed/14736746

        19. Hanada R, Teranishi H, Pearson JT, Kurokawa M, Hosoda H, Fukushima N, Fukue Y, Serino R, Fujihara H, Ueta Y, Ikawa M, Okabe M, et al.
          Neuromedin U has a novel anorexigenic effect independent of the leptin signaling pathway.
          Nat Med 2004; 10:1067-1073.
          http://www.ncbi.nlm.nih.gov/pubmed/15448684

        20. Tergaonkar V, Bottero V, Ikawa M, Li Q, Verma IM.
          IkappaB kinase-independent IkappaBalpha degradation pathway: functional NF-kappaB activity and implications for cancer therapy.
          Mol Cell Biol. 2003; 23(22):8070-83.
          http://www.ncbi.nlm.nih.gov/pubmed/14585967

        21. Ikawa M, Tanaka N, Kao WW, Verma IM.
          Generation of transgenic mice using lentiviral vectors: a novel preclinical assessment of lentiviral vectors for gene therapy.
          Mol Ther. 2003; 8(4):666-73.
          http://www.ncbi.nlm.nih.gov/pubmed/14529840

        22. Kimura T, Ito C, Watanabe S, Takahashi T, Ikawa M, Yomogida K, Fujita Y, Ikeuchi M, Asada N, Matsumiya K, Okuyama A, Okabe M, et al.
          Mouse germ cell-less as an essential component for nuclear integrity.
          Mol Cell Biol 2003; 23:1304-1315.
          http://www.ncbi.nlm.nih.gov/pubmed/12556490

        23. Inoue N, Ikawa M, Nakanishi T, Matsumoto M, Nomura M, Seya T, Okabe M.
          Disruption of Mouse CD46 Causes an Accelerated Spontaneous Acrosome Reaction in Sperm.
          Mol Cell Biol 2003; 23:2614-2622.
          http://www.ncbi.nlm.nih.gov/pubmed/12640142

        24. Tiscornia G, Singer O, Ikawa M, Verma IM.
          A general method for gene knockdown in mice by using lentiviral vectors expressing small interfering RNA.
          Proc Natl Acad Sci U S A. 2003; 100(4):1844-8.
          http://www.ncbi.nlm.nih.gov/pubmed/12552109

        25. Yamagata K, Nakanishi T, Ikawa M, Yamaguchi R, Moss SB, Okabe M.
          Sperm from the calmegin-deficient mouse have normal abilities for binding and fusion to the egg plasma membrane.
          Dev Biol 2002; 250:348-357.
          http://www.ncbi.nlm.nih.gov/pubmed/12376108

        26. Nakanishi T, Kuroiwa A, Yamada S, Isotani A, Yamashita A, Tairaka A, Hayashi T, Takagi T, Ikawa M, Matsuda Y, Okabe M.
          FISH Analysis of 142 EGFP Transgene Integration Sites into the Mouse Genome.
          Genomics 2002; 80:564-574.
          http://www.ncbi.nlm.nih.gov/pubmed/12504848

        27. Baba D, Kashiwabara S, Honda A, Yamagata K, Wu Q, Ikawa M, Okabe M, Baba T.
          Mouse Sperm Lacking Cell Surface Hyaluronidase PH-20 Can Pass through the Layer of Cumulus Cells and Fertilize the Egg.
          J Biol Chem 2002; 277:30310-30314.
          http://www.ncbi.nlm.nih.gov/pubmed/12065596

        28. Ikawa M, Tergaonkar V, Ogura A, Ogonuki N, Inoue K, Verma IM.
          Restoration of spermatogenesis by lentiviral gene transfer: offspring from infertile mice.
          Proc Natl Acad Sci U S A. 2002; 99(11):7524-9.
          http://www.ncbi.nlm.nih.gov/pubmed/12032316

        29. Pfeifer A, Ikawa M, Dayn Y, Verma IM.
          Transgenesis by lentiviral vectors: lack of gene silencing in mammalian embryonic stem cells and preimplantation embryos.
          Proc Natl Acad Sci U S A. 2002; 99(4):2140-5.
          http://www.ncbi.nlm.nih.gov/pubmed/11854510

        30. Yamada M, Miyagawa S, Matsunami K, Koresawa Y, Kurihara T, Ikawa M, Okabe M, Shirakura R.
          A codon exchange DAF is a powerful material for xenografting.
          Transplant Proc 2001; 33:744-745.
          http://www.ncbi.nlm.nih.gov/pubmed/11267049

        31. Nakanishi T, Ikawa M, Yamada S, Toshimori K, Okabe M.
          Alkalinization of Acrosome Measured by GFP as a pH Indicator and Its Relation to Sperm Capacitation.
          Dev Biol 2001; 237:222-231.
          http://www.ncbi.nlm.nih.gov/pubmed/11518518

        32. Miyagawa S, Yamada M, Matsunami K, Koresawa Y, Ikawa M, Okabe M, Shirakura R.
          A synthetic dDAF (CD55) gene based on optimal codon usage for transgenic animals.
          J Biochem 2001; 129:795-801.
          http://www.ncbi.nlm.nih.gov/pubmed/11328604

        33. Kitao Y, Ozawa K, Miyazaki M, Tamatani M, Kobayashi T, Yanagi H, Okabe M, Ikawa M, Yamashima T, Stern DM, Hori O, Ogawa S.
          Expression of the endoplasmic reticulum molecular chaperone (ORP150) rescues hippocampal neurons from glutamate toxicity.
          J Clin Invest 2001; 108:1439-1450.
          http://www.ncbi.nlm.nih.gov/pubmed/11714735

        34. Kimura SH, Ikawa M, Ito A, Okabe M, Nojima H.
          Cyclin G1 is involved in G2/M arrest in response to DNA damage and in growth control after damage recovery.
          Oncogene 2001; 20:3290-3300.
          http://www.ncbi.nlm.nih.gov/pubmed/11423978

        35. Ikawa M, Nakanishi T, Yamada S, Wada I, Kominami K, Tanaka H, Nozaki M, Nishimune Y, Okabe M.
          Calmegin is required for fertilin alpha/beta heterodimerization and sperm fertility.
          Dev Biol 2001; 240:254-261.
          http://www.ncbi.nlm.nih.gov/pubmed/11784061

        36. Horie K, Kuroiwa A, Ikawa M, Okabe M, Kondoh G, Matsuda Y, Takeda J.
          Efficient chromosomal transposition of a Tc1/mariner- like transposon Sleeping Beauty in mice.
          Proc Natl Acad Sci U S A 2001; 98:9191-9196.
          http://www.ncbi.nlm.nih.gov/pubmed/11481482 http://www.pnas.org/cgi/content/full/98/16/9191 http://www.ncbi.nlm.nih.gov/pubmed/11481482

        37. Shi W, Kumanogoh A, Watanabe C, Uchida J, Wang X, Yasui T, Yukawa K, Ikawa M, Okabe M, Parnes JR, Yoshida K, Kikutani H.
          The class IV semaphorin CD100 plays nonredundant roles in the immune system : Defective B and T cell activation in CD100-deficient mice.
          Immunity 2000; 13:633-642.
          http://www.ncbi.nlm.nih.gov/pubmed/11114376

        38. Koresawa Y, Miyagawa S, Ikawa M, Matsunami K, Yamada M, Shirakura R, Okabe M.
          Synthesis of a New Cre Recombinase Gene Based on Optimal Codon Usage for Mammalian Systems.
          J Biochem (Tokyo) 2000; 127:367-372.
          http://www.ncbi.nlm.nih.gov/pubmed/10731707

        39. Koresawa Y, Miyagawa S, Ikawa M, Matsunami K, Yamada M, Okabe M, Shirakura R.
          A new Cre recombinase gene based on optimal codon usage in mammals: a powerful material for organ-specific gene targeting.
          Transplant Proc 2000; 32:2516-2517.
          http://www.ncbi.nlm.nih.gov/pubmed/11120274

        40. Keng VW, Yagi H, Ikawa M, Nagano T, Myint Z, Yamada K, Tanaka T, Sato A, Muramatsu I, Okabe M, Sato M, Noguchi T.
          Homeobox gene hex is essential for onset of mouse embryonic liver development and differentiation of the monocyte lineage.
          Biochem Biophys Res Commun 2000; 276:1155-1161.
          http://www.ncbi.nlm.nih.gov/pubmed/11027604

        41. Hashimoto H, Shintani N, Nishino A, Okabe M, Ikawa M, Matsuyama S, Itoh K, Yamamoto K, Tomimoto S, Fujita T, Hagihara N, Mori W, et al.
          Mice with markedly reduced PACAP (PAC(1)) receptor expression by targeted deletion of the signal peptide.
          J Neurochem 2000; 75:1810-1817.
          http://www.ncbi.nlm.nih.gov/pubmed/11032869

        42. Ono K, Takii T, Onozaki K, Ikawa M, Okabe M, Sawada M.
          Migration of exogenous immature hematopoietic cells into adult mouse brain parenchyma under GFP-expressing bone marrow chimera.
          Biochem Biophys Res Commun 1999; 262:610-614.
          http://www.ncbi.nlm.nih.gov/pubmed/10471372

        43. Nakanishi T, Ikawa M, Yamada S, Parvinen M, Baba T, Nishimune Y, Okabe M.
          Real-time observation of acrosomal dispersal from mouse sperm using GFP as a marker protein.
          FEBS Lett 1999; 449:277-283.
          http://www.ncbi.nlm.nih.gov/pubmed/10338148

        44. Kawakami N, Sakane N, Nishizawa F, Iwao M, Fukada SI, Tsujikawa K, Kohama Y, Ikawa M, Okabe M, Yamamoto H.
          Green fluorescent protein-transgenic mice: immune functions and their application to studies of lymphocyte development.
          Immunol Lett 1999; 70:165-171.
          http://www.ncbi.nlm.nih.gov/pubmed/10656669

        45. Kawakami N, Nishizawa F, Sakane N, Iwao M, Tsujikawa K, Ikawa M, Okabe M, Yamamoto H.
          Roles of integrins and CD44 on the adhesion and migration of fetal liver cells to the fetal thymus.
          J Immunol 1999; 163:3211-3216.
          http://www.ncbi.nlm.nih.gov/pubmed/10477589

        46. Kato M, Yamanouchi K, Ikawa M, Okabe M, Naito K, Tojo H.
          Efficient selection of transgenic mouse embryos using EGFP as a marker gene.
          Mol Reprod Dev 1999; 54:43-48.
          http://www.ncbi.nlm.nih.gov/pubmed/10423296

        47. Harada YN, Shiomi N, Koike M, Ikawa M, Okabe M, Hirota S, Kitamura Y, Kitagawa M, Matsunaga T, Nikaido O, Shiomi T.
          Postnatal growth failure, short life span, and early onset of cellular senescence and subsequent immortalization in mice lacking the xeroderma pigmentosum group G gene.
          Mol Cell Biol 1999; 19:2366-2372.
          http://www.ncbi.nlm.nih.gov/pubmed/10022922

        48. Hamada Y, Kadokawa Y, Okabe M, Ikawa M, Coleman JR, Tsujimoto Y.
          Mutation in ankyrin repeats of the mouse notch2 gene induces early embryonic lethality.
          Development 1999; 126:3415-3424.
          http://www.ncbi.nlm.nih.gov/pubmed/10393120

        49. Baumstark-Khan C, Palm M, Wehner J, Okabe M, Ikawa M, Horneck G.
          Green Fluorescent Protein (GFP) as a Marker for Cell Viability After UV Irradiation.
          Journal of Fluorescence 1999; 9:37-43. http://www.springerlink.com/content/x148vh92v22u82k6/

        50. Mikata S, Miyagawa S, Yoshitatsu M, Ikawa M, Okabe M, Matsuda H, Shirakura R.
          Prevention of hyperacute rejection by phosphatidylinositol-anchored mini-complement receptor type 1.
          Transpl Immunol 1998; 6:107-110.
          http://www.ncbi.nlm.nih.gov/pubmed/9777699

        51. Matsuki M, Yamashita F, Ishida-Yamamoto A, Yamada K, Kinoshita C, Fushiki S, Ueda E, Morishima Y, Tabata K, Yasuno H, Hashida M, Iizuka H, et al.
          Defective stratum corneum and early neonatal death in mice lacking the gene for transglutaminase 1 (keratinocyte transglutaminase).
          Proc Natl Acad Sci U S A 1998; 95:1044-1049.
          http://www.ncbi.nlm.nih.gov/pubmed/9448282

        52. Hadjantonakis AK, Gertsenstein M, Ikawa M, Okabe M, Nagy A.
          Generating green fluorescent mice by germline transmission of green fluorescent ES cells.
          Mech Dev 1998; 76:79-90.
          http://www.ncbi.nlm.nih.gov/pubmed/9867352

        53. Hadjantonakis A, Gertsenstein M, Ikawa M, Okabe M, Nagy A.
          Non-invasive sexing of preimplantation stage mammalian embryos.
          Nat Genet 1998; 19:220-222.
          http://www.ncbi.nlm.nih.gov/pubmed/9662390

        54. Tarutani M, Itami S, Okabe M, Ikawa M, Tezuka T, Yoshikawa K, Kinoshita T, Takeda J.
          Tissue-specific knockout of the mouse Pig-a gene reveals important roles for GPI-anchored proteins in skin development.
          Proc Natl Acad Sci U S A 1997; 94:7400-7405.
          http://www.ncbi.nlm.nih.gov/pubmed/9207103

        55. Tanaka H, Ikawa M, Tsuchida J, Nozaki M, Suzuki M, Fujiwara T, Okabe M, Nishimune Y.
          Cloning and characterization of the human Calmegin gene encoding putative testis-specific chaperone.
          Gene 1997; 204:159-163.
          http://www.ncbi.nlm.nih.gov/pubmed/9434179

        56. Okabe M, Ikawa M, Kominami K, Nakanishi T, Nishimune Y.
          'Green mice' as a source of ubiquitous green cells.
          FEBS Lett 1997; 407:313-319.
          http://www.ncbi.nlm.nih.gov/pubmed/9175875

        57. Miyagawa S, Mikata S, Tanaka H, Ikawa M, Kominami K, Seya T, Nishimune Y, Shirakura R, Okabe M.
          The regulation of membrane cofactor protein (CD46) expression by the 3' untranslated region in transgenic mice.
          Biochem Biophys Res Commun 1997; 233:829-833.
          http://www.ncbi.nlm.nih.gov/pubmed/9168942

        58. Miyagawa S, Ikawa M, Kominami K, Tanaka H, Mikata S, Matsuda H, Seya T, Shirakura R, Okabe M.
          The regulation of membrane cofactor protein (CD46) expression in transgenic mice: the importance of the first 125 BP of the 3' untranslated region.
          Transplant Proc 1997; 29:941-942.
          http://www.ncbi.nlm.nih.gov/pubmed/9123597

        59. Ikawa M, Wada I, Kominami K, Watanabe D, Toshimori K, Nishimune Y, Okabe M.
          The putative chaperone calmegin is required for sperm fertility.
          Nature 1997; 387:607-611.
          http://www.ncbi.nlm.nih.gov/pubmed/9177349

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